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large aggregates purpurin  (Chem Impex International)


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    Chem Impex International large aggregates purpurin
    Large Aggregates Purpurin, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/large aggregates purpurin/product/Chem Impex International
    Average 95 stars, based on 1 article reviews
    large aggregates purpurin - by Bioz Stars, 2026-02
    95/100 stars

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    Figure 3. Changes in gene expression in 2D cultured porcine kidney organoids. (A) Expression of special markers in different periods. The specific markers for PS cells (MIXL1), IM cells (PAX2), MM cells (SIX2 and PAX2), and UE cells (SIX2 and PAX2) were discovered in the corresponding porcine kidney organoids on days 3, 7, and 14 of 2D culture by immunofluorescence test (MIXL1, PAX2, and SIX2 were all localized to the nucleus) (scale bar: 100 µm). (B) Expression level changes in renal progenitor cell markers at 4 time points (day 0, 7, 14, and 21) of the 2D cultured porcine kidney organoids. The relative expression levels of markers about early nephrons (JAG1, BMP2), nephrogenic stroma (SALL1, ITGA8), ureteral buds (VLDLR, SLCO4C1), and endothelial progenitor cells (KDR, ANGPT2) were measured by real-time PCR. (C) Expression level changes in mature nephron components markers at 4 time points (day 0, 7, 14, and 21) of the 2D cultured porcine kidney organoids. The relative expression levels of markers about podocytes (NPHS1, MAFB), proximal tubules (AQP1, HNF1B), distal tubules (ALDLH1), and endothelial cells (CD31) were measured by real-time PCR. Each value was averaged from three replicates and presented as mean ± SD. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant.

    Journal: International journal of molecular sciences

    Article Title: Porcine Kidney Organoids Derived from Naïve-like Embryonic Stem Cells.

    doi: 10.3390/ijms25010682

    Figure Lengend Snippet: Figure 3. Changes in gene expression in 2D cultured porcine kidney organoids. (A) Expression of special markers in different periods. The specific markers for PS cells (MIXL1), IM cells (PAX2), MM cells (SIX2 and PAX2), and UE cells (SIX2 and PAX2) were discovered in the corresponding porcine kidney organoids on days 3, 7, and 14 of 2D culture by immunofluorescence test (MIXL1, PAX2, and SIX2 were all localized to the nucleus) (scale bar: 100 µm). (B) Expression level changes in renal progenitor cell markers at 4 time points (day 0, 7, 14, and 21) of the 2D cultured porcine kidney organoids. The relative expression levels of markers about early nephrons (JAG1, BMP2), nephrogenic stroma (SALL1, ITGA8), ureteral buds (VLDLR, SLCO4C1), and endothelial progenitor cells (KDR, ANGPT2) were measured by real-time PCR. (C) Expression level changes in mature nephron components markers at 4 time points (day 0, 7, 14, and 21) of the 2D cultured porcine kidney organoids. The relative expression levels of markers about podocytes (NPHS1, MAFB), proximal tubules (AQP1, HNF1B), distal tubules (ALDLH1), and endothelial cells (CD31) were measured by real-time PCR. Each value was averaged from three replicates and presented as mean ± SD. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant.

    Article Snippet: The primary antibodies included OCT4 (Santa Cruz Biotechnology, Dallas, TX, USA), SOX2 (Cell Signaling Technology, Danvers, MA, USA), KLF4 (Cell Signaling Technology), C-MYC (Cell Signaling Technology), LHX1 (GeneTex, Alton PkwyIrvine, CA, USA), MIXL1 (proteintech, Chicago, IL, USA), PAX2 (Santa Cruz Biotechnology), and SIX2 (Abcam, Waltham, MA, USA).

    Techniques: Gene Expression, Cell Culture, Expressing, Real-time Polymerase Chain Reaction